Regeneration of Mycelial Protoplasts ofAspergillus oryzae
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چکیده
منابع مشابه
SHOOT REGENERATION FROM PROTOPLASTS OF PENNISETUM AMERICANUM L (MIL CEREAL)
A finely dispersed cell suspension was obtained from embryogenic calli of two genotypes of pearl millet (Pennisetum americanum L. Mil cereal). Protoplasts isolated from both cell suspensions divided and produced globular embryoids and calli. On an agar medium, the embryoids were induced to form albino shoots or albino plantlet- like structures
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Both mycelial fragments and protoplasts were successfully employed for mutagenesis of Micromonospora rosaria NRRL 3718, and the results were compared. The optimal conditions and effective procedures for mutagenesis of M. rosaria by a chemical mutagen, N-methyl-N'-nitro-N-nitrosoguanidine, have been determined. Mutation was efficiently induced when mycelial fragments were treated with N-methyl-N...
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Saffron (Crocus sativus L.) protoplasts were isolated from the cells of a suspension culture or calli with a solution of Cellulase, Pectinase and Hemicellulase and embedded in Ca-alginate beads. They were cultured with or without nurse cells in MS medium supplemented with 2,4-D and 6-benzylaminopurine at 25°C. After several changes of medium, cell-clusters appeared on the surface of the Ca-algi...
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This article describes how to obtain isolated cells with no surrounding cell wall by enzymatic digestion of Ectocarpus filaments. The resultant protoplasts are totipotent and regenerate to produce individual algae under appropriate culture conditions. The yield of protoplasts and their capacity to regenerate are highly dependent on the Ectocarpus strain used, the stage of the life cycle, and th...
متن کاملplant regeneration from protoplasts of lilium ledebourii (baker) boiss
for half a century, the limitations of obtaining cross-combinations in lilies because of the incompatibility and incongruity between different varieties have been known. somatic hybridization is one of the most powerful tools for achieving distant interspecific hybrids. for this purpose, protoplast preparation is a first and important step in efficient system for the regeneration of plants from...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1982
ISSN: 0002-1369
DOI: 10.1080/00021369.1982.10865148